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Lab projects in Salmonella |
Interaction of bacterial effectors with targets in epithelial cells, macrophages and lymphocytes. |
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We are currently examining effectors of the SPI-2 system (see Figure 3 right) for
their effects on sterol recruitment, metabolism and bacterial virulence in the mouse model. |
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Recruitment of host sterol by S. Typhimurium. |
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Up to 30-40% of total host cholesterol is diverted to the SCV and our efforts focus
on its source and mechanisms of trafficking to the SCV (see Figure 4 right). |
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Perturbation of the host sterol metabolism.
We are investigating the requirement for non-sterol precursors in protecting infected cells from
apoptotic and/or necrotic death.
Salmonella microarrays and other gene screens.
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These are employed to identify subsets of S. typhimurium virulence
determinants required for intracellular bacterial replication (see Figure 5 right). |
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